Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma – Ocular Hypertension offers a detailed, step-by-step protocols for isolating, characterizing, and analyzing cells and tissues of the eye's anterior segment-where OHT originates-using techniques from molecular biology, pharmacology, and histology.
Across eight thematic sections, the book covers ocular anatomy, drug screening, receptor localization, cell-based assays, in vitro and in vivo models, drug formulation, and cutting-edge technologies such as gene editing, single-cell RNA sequencing, and stem cell therapies. Each protocol is designed to support reproducibility and translational relevance.
Key Features:
-Details validated protocols for drug discovery and disease modeling
-Demonstrates methods for studying receptor function and pharmacokinetics
-Provides animal models and ex vivo systems to evaluate therapeutic strategies
-Incorporates emerging tools such as CRISPR, metabolomics, and exosomes
-Bridges experimental design with clinical relevance for glaucoma research
